Peptide Reconstitution & Concentration Calculator

For Research Purposes

Peptide Reconstitution & Concentration Calculator

Accurate, research-only calculations for lyophilised peptides and solution volumes.

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Select your syringe size

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Select peptide amount (mg)

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Required sample size

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Mixing solution to add

📖 Peptide calculator info

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This free peptide calculator helps researchers work out reconstitution and measurement in the lab. Enter your vial amount in mg, your mixing water in mL and a required sample size in mcg or mg. The tool returns the volume to measure and graduated units for common U-100 laboratory syringes.

Also called a peptide reconstitution calculator or peptide dilution calculator, it converts mg↔mL and mcg↔IU and suggests easy measurement points for typical vials.

🔢 Quick conversions

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1 mL = 100 IU
1 mg = 1000 mcg
1 IU = 0.01 mL

💡 Peptide reconstitution examples

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Example 1: 10mg peptide vial

Scenario: 10mg peptide, want 250 mcg sample

Recommendation: Add 2mL bacteriostatic water

Result: Measure 0.05mL (5 IU) for ~250 mcg sample

Example 2: 5mg peptide vial

Scenario: 5mg peptide, want 500 mcg sample

Recommendation: Add 1mL bacteriostatic water

Result: Measure 0.1mL (10 IU) for ~500 mcg sample

How do I reconstitute lyophilised peptides?

Add bacteriostatic water slowly to your peptide vial. Use the calculator to determine the volume needed for your intended concentration. For in vitro research only.

What syringe size is shown?

U-100 graduated syringes, selectable as 0.3 mL, 0.5 mL, or 1 mL.

How do I convert mg to mcg?

1 mg equals 1000 mcg. The calculator keeps both in sync.

What's the difference between mL and IU?

mL is volume. IU are unit markings on U-100 graduated syringes where 1 mL equals 100 IU.